MiR-557 downregulation lowered cell proliferation and malignancy in glioblastoma by targeting ADAM17


 Purpose: To examine the role of miR-557 in pathogenesis and development glioblastoma (GBM) its potential regulatory mechanism.
 Methods: Dysregulation GBM cells was determined using quantitative (real-time) PCR (qPCR). Cell proliferative ability, migratory activity invasive ability were measured MTT colorimetric assay, wound healing assay Transwell chambers assay. The direct target mRNA predicted, further validated TargetScan luciferase respectively.
 Results: Compared with human astrocytes (HAs), downregulation observed cell lines. In U-251MG A172 that overexpressed mimics, level proliferation significantly reduced, width larger, number invaded wan decreased than NC mimics. Predictive results from demonstrated directly targets 3’-untranslated region (3’-UTR) ADAM17. cells, upregulation E-cadherin (E-cad) N- cadherin (N-cad) vimentin (Vim) caused by mimics as compared Expression ADAM17, NICD, HES1, EGFR downregulated upregulated inhibitors cells.
 Conclusion: Downregulation accelerates growth malignancy targeting ADAM17 3’-UTR, providing a prognostic therapeutic factor for new drug discovery GBM.